eDossier
Graduate Science Course Work
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BEC 563 - Fermentation of Recombinant Microorganisms
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Upon completion of this course, I gained the skills to perform small-scale fermentations using recombinant microorganisms and develop a comprehensive understanding of process optimization techniques. With a focus on protein production, I explored the factors influencing gene expression and recombinant protein yield.
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MB 714 - Microbial Metabolic Regulation
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An integrative perspective on bacterial physiology and metabolism through analysis of metabolic regulatory functions.
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BIT 510 - Core Technologies in Molecular and Cellular Biology
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Basic technologies of recombinant DNA procedures, gene expression, isolation and identification of nucleic acids and proteins.
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MB 585 - Industry Case Studies in Microbial Biotechnology
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Project-based course directly working with biotechnology and pharmaceutical companies. Students work in teams on a company-specific project. Written and oral communication skills as well as teamwork, flexibility, and ambiguity management are emphasized.
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Techniques
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Small-scale fermentation techniques
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Cultivation of recombinant microorganisms
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Optimization methods for fermentation processes
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Analysis of factors influencing gene expression
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Evaluation of recombinant protein yield
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Techniques for monitoring and controlling fermentation parameters
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Troubleshooting common issues in fermentation processes
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Experimental design and data analysis in fermentation studies
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Application of statistical methods in process optimization
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Use of specialized equipment for fermentation experiments.
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Isolation and Purification of Plasmid DNA and Plasmid Quantitation
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PCR amplification
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Column-based purification of DNA fragments
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Gibson assembly and bacterial transformation
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PCR Screening
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Gel electrophoresis
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SDS-PAGE
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Western blot
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Affinity chromatography
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Bradford assay